:: Course pre-event: May 27, 2012
:: Workshop: May 28 to 30, 2012
Preliminary Program
May 27 | Course | 09:00am to 07:00pm
1. Chaperone concepts
- (9-10:30am): HARM KAMPINGA
. Basic chaperone
reactions and experimental systems to measure such activities
. Why cells need
chaperones
. Induced resistance
(thermotolerance: just phenotypical, excluding HSF-1 regulation)
. HSP families and
functional diversity
.
Folding-remodeling-proteasomal degradation-autophagosomal degradation
10:30am-11:00am Coffee break
2. Heat shock responses-Mechanisms of HSP Stress responses - (11:00am-12:00am): LEA SISTONEN
. HSF mediated HSP regulation
. Insuline-GF pathway as major regulator of HSF (link with Robert)
. Phenotypes of HSF-knockouts?
. HSP/HSR: development & ageing - (12:00am-1:00pm): LARRY HIGHTOWER
. differential regulation of HSP in development
. declined HS response in aging cells
. effects HSP upregulation on longevity
1:00pm-2:15pm LUNCH BREAK
3. The Stress response and disease
. HSP/HSR: cardiovascular disease - (2:15pm-3:15pm): IVOR BENJAMIN
. Role of HSP in ischemia/reperfusion
. Role of HSP in redox status and heart disease
. Role of HSP in AF?
. HSP mutations leading to cardiac diseases
. HSP knockouts and heart diseases
. HSP/HSR: cancer - (3:15pm-4:15pm): DANIEL CIOCCA
. Upregulated HSP in cancers
. Functionality of HSP in cancer cell resistance
. HSPs as predictors for (treatment) outcome in human cancers
. HSPs as therapeutic targets in (human) cancer
4:15pm-4:45pm Coffe break
. UPR mechanisms : role in development & disease - (4:45pm-5:45pm): LINDA HENDERSHOT
. Explanation of ER folding machine of the cells
. The 3 branches of the UPR
. ER chaperones: folding and ERAD
. ER/UPR and disease
. Protein aggregation diseases - (5:45pm-6:45pm) : HARM KAMPINGA
. Protein aggregation as cause for neurodegenerative diseases
. Role of protein quality control/HSPs in suppression of protein aggregation diseases
. HSP mutations leading to neurodegenerative disease
7:00pm-8:00pm Light
bites and cocktails with posters
May 28 | 09:00 a.m. to 08:00 p.m.
09:00 a.m. Opening Remarks | Cristina Bonorino (BRA) (5 min) | Antonio De Maio (USA) (20min)
1.Stress Responses in the Nervous System I (20
min each senior, 15 min discussion)
Chairs: Ian R. Brown (CAN) and Maria Estela Andres (CHI)
:: Vilma R. Martins (BRA) | Interaction of stress-inducible protein 1 with prion protein triggers neuroprotection
:: Soledad Matus Montero (CHI) | Protein folding stress in neurodegenerative disease
:: Ian R. Brown (CAN) | Expression of heat shock proteins, including Hsp70B' in differentiated human neuronal cells
10:40 a.m. - 10:55 a.m. – Break
10:55 a.m. - 11:50 a.m.
Stress Responses in
the Nervous System II (20 min each senior, 15 min
discussion)
:: Maria Estela Andres (CHI) | Epigenetic mechanism controlling hsp70 expression in neurons
:: Hugo Peluffo (UY) | Regulation of neuronal survival by the CD300f immunoreceptor in the normal and injured brain
11:50 p.m. - 01:30 p.m Lunch Break
1:30 p.m. - 2:45 p.m
2. Transcription factors in heat shock responses (20
min each senior, 15 min discussion)
Chair: Lea Sistonen (FIN)
:: Gabriella Santoro (ITA) | HSF1 at the crossroads of inflammatory and survival signaling
:: Lea Sistonen (FIN) | Stress-inducible post-translational modifications regulating mammalian heat shock factors
:: Ayesha Murshid (USA) | Protein kinase A regulates molecular chaperone
transcription and protein aggregation
02:45 p.m. - 04:15 p.m.
3. Stress proteins and microbiology
(20 min each senior, 10 min discussion)
Chair: Iara Linhares (BRA)
:: Steve Witkin (USA) | Heat Shock Protein Research in Women’s Health
:: Iara Linhares (BRA) | HSPs and infections in the reproductive tract of women in Brazil
:: Manuel Alejandro Barbieri (USA) | Inhibition of Rab5 activation
during Live Pseudomonas-macrophage interaction
:: Wolfgang Schumann (GER) | Role of the Bacillus subtilis FtsH metalloprotease during initiation of
sporulation
04:15 p.m. - 04:40 p.m. Break
04:40 p.m - 06:00 p.m.
Chair: Ivor Benjamin (USA)
:: Ivor Benjamin (USA) | Redox Stress Response Pathways: A Balancing Act with Rhythmic Moves
:: Claudina Rodrigues-Pousada (PT) | Arsenic exposure induces iron deficiency and destabilizes the high-affinity iron uptake system
:: Magnus Ake Gidlund (BRA) | Stress response and atherosclerosis: How should
we measure it?
:: Talk
selected from abstracts - Norma Possa Marroni (BRA) | Glutamine
Antioxidant Properties and its Role in VEGF-Akt Pathways in Portal Hypertension
Gastropathy
06:00 p.m. - 07:30 p.m. Poster
sessions with tea and cookies
May 29 | 09:00 a.m. to 07:00 p.m.
09:00 a.m. - 10:20 a.m.
5. Chaperones I (20
min each senior, 10 min abstract talk, 10 min discussion)
Chair: Maristela Camargo (BRA)
:: Harm Kampinga (NED) | Role of chaperones in the routing of misfolded or aggregated proteins towards degradation
:: Carlos Ramos (BRA) | Investigation of the Structure-Function Relationship of Human, Yeast and Sugar Cane Molecular Chaperones by Biophysical Tools
:: Linda Hendershot (USA) | ER Chaperones
:: Talk
selected from abstracts - Mariana Borsa (BRA) | HIV promotes UPR activation
through ATF6 pathway in cells from HIV-positive patients under different
antiretroviral therapies
10:20 a.m. - 10:40 a.m. - Break
Chaperones
II (20 min each senior, 10 min abstract talk, 10 min discussion)
:: Vince Guerriero (USA) | HspBP1, a Co-chaperone with Multiple Functions
:: Maristela Camargo (BRA) | Dysfunctional Unfolded Protein Response as a cause of hypogammaglobulinemia
:: Maristela Camargo (BRA) | Dysfunctional Unfolded Protein Response as a cause of hypogammaglobulinemia
:: Mario Daniel Galigniana (AR) | Regulation of Steroid Receptor Function by Hsp90-Binding Immunophilins. A Potential New Platform for Drug Treatment
:: Talk
selected from abstracts - Fernanda M. C. Sodré (BRA) | Defective Response to
ER Stress due to mutated BIP as a probable cause for Hypogammaglobulinemia
12:00 a.m. - 1:30 p.m. Lunch Break
1:30 p.m. - 2:40 p.m.
6. HSP and cancer I (20
min each senior, 10 min discussion)
Chair: José Alexandre Barbuto (BRA)
Chair: José Alexandre Barbuto (BRA)
:: Daniel Ciocca (ARG) | Interrelations between Caveolin-1 and Heat shock proteins in cancer
:: Pramod Srivastava (USA) | Hsp90 helps sequester antigenic epitopes long after the antigen is gone
:: Maria Bausero (UY) | Nanotechnologies and the development of anti-cancer HSP's vaccines
2:40
p.m. – 2:50 p.m. Short Break
2:50 p.m. – 4:00 p.m.
HSP
and cancer II (20 min each senior, 10 min abstract talk, 10 min discussion)
:: Mathias Gehrmann (GER) | Lipid association of membrane-bound Hsp70 and its implication in tumor therapy
:: José Alexandre Barbuto (BRA) | HSP and dendritic cell maturation in cancer patients
:: Talk selected from abstracts -
Gisela N Castro (ARG) | Hsp27 (HSPB1): a possible surrogate molecular marker
for loss of heterozigocity (LOH) of chromosome 1p in oligodendrogliomas.
Correlation with histopathologic grade, Hsp70 (HSPA), PCNA, beta-catenin, p53
and MGMT
:: Talk selected from abstracts
- Daiana G. Alvarez-Olmedo (ARG) | Effects of cadmium on the expression of
β-catenin, caveolin-1 and HSPs in ERα-positive and ERα-negative cancer cell
lines
4:00 p.m.
– 4:30 p.m. Coffee Break
4:30 p.m. - 6:00 p.m.
7. Hsps and Immune tolerance (20
min each senior, 10 min abstract talk, 10 min discussion)
Chair: Verônica Coelho (BRA) and Kamal Moudgil (USA)
:: Willem van Eden (NED) | Stress proteins are targets for inflammatory disease suppressive long lived antigen specific Foxp3+ Treg
:: Verônica Coelho (BRA) | Strategies
to identify immunoregulatory Hsp60 peptides
:: Kamal Moudgil (USA) | Modulation of autoimmunity by cytokine responses and immune tolerance against HSP65
:: Talk selected from abstracts - Thiago
J. Borges (BRA) | Mechanisms of inhibition of acute graft rejection induced by
mycobacterial Hsp70
:: Talk selected from abstracts - Ana
Cristina Gomes-Santos (BRA) | Hsp65-producing Lactococcus lactis prevents intestinal inflammatory disease via
IL-10 and TLR2 dependent pathways
6:00 p.m. - 7:00 p.m. Poster sessions
May 30 | 09:00 a.m. to 11:30am
8. Cell stress and inflamation (20 min each senior, 10 min abstract talk, 10 min discussion)
Chair: Elizabeth Repasky
(USA)
:: Cesar Fraga (ARG) | Inflammation and Flavonoids
:: Elizabeth Repasky (USA) | Role of mild thermal stress on neutrophil homeostasis and cytokine activity following radiation
:: John Williams (UK) | HSPA1A and inflammatory responses –the beginning
and the end
:: Talk
selected from abstracts - Sylvia Stella Amaral (BRA) | Increased ATP/ADP signaling amplifies
acetaminophen toxicity
Closing conference
Chair: Cristina Bonorino (BRA)
Larry
Hightower (CAN) | Hyperbaric Oxygen Therapy: Cytoprotection and Stress
Conditioning
11:00 a.m. Closing Remarks
